Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
نویسندگان
چکیده
منابع مشابه
Hydrodynamic stress in orbitally shaken bioreactors
Materials and methods CHO-DG44 cells were cultivated in suspension in 1-L bottles as described in [1]. To determine conditions under which the shear stress was harmful for the cells, the bottles were orbitally shaken on an ES-X platform (Kühner AG, Birsfelden, Switzerland) at agitation rates from 150 to 200 rpm for 24 h. Control cultures were run in parallel with agitation at 110 rpm. The veloc...
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Mammalian cell cultures have become a major topic of research in the biopharmaceutical industry. This kind of cells require specific conditions to grow. In this thesis, we study the hydrodynamics of orbitally shaken reactors (OSR), a recently introduced kind of bioreactors for mammalian cell cultures that represents a simple to operate and cheap alternative to commonly used reactors such as sti...
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Using the basic principles of molecular biology and laboratory techniques presented in this chapter, researchers should be able to create a wide variety of AAV vectors for both clinical and basic research applications. Basic vector design concepts are covered for both protein coding gene expression and small non-coding RNA gene expression cassettes. AAV plasmid vector backbones (available via A...
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ژورنال
عنوان ژورنال: Molecular Therapy - Methods & Clinical Development
سال: 2019
ISSN: 2329-0501
DOI: 10.1016/j.omtm.2018.11.004